Chromatin Immunoprecipitation:Article Title: Endophilin-A3 and Galectin-8 control the clathrin-independent endocytosis of CD166
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology Animals and other organisms Human research participants Clinical data Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used The following antibodies were purchased from the indicated suppliers: mouse monoclonal anti-CD166 clone 3A6 (Bio-Rad, MCA1926, 1:200 for immunofluorescence and flow cytometry on human cell lines); goat polyclonal anti-CD166 (R&D Systems, AF1172, 1:20 for immunofluorescence on mouse cell lines); mouse monoclonal anti-CD166 clone B-6 (Santa Cruz Biotechnology, sc-74558, 1:500 for Western blotting); mouse monoclonal anti-TfR (BD Biosciences, 555534, 1:1,000 for Western blotting); rabbit polyclonal anti-α-adaptin (Santa Cruz Biotechnology, sc-10761, 1:1,000 for Western blotting); rabbit monoclonal anti-EGFR (Cell Signaling Technology, 4267, 1:4,000 for Western blotting); rabbit monoclonal anti-endoA1 (Cell Signaling Technology, 3 nature research | reporting sum m ary O ctober 2018 65469, 1:100 for immunofluorescence and 1:1,000 for Western blotting); mouse monoclonal anti-endoA2 (Santa Cruz Biotechnology, sc-365704, 1:50 for immunofluorescence and 1:500 for Western blotting); mouse monoclonal anti-endoA2 coupled to Alexa Fluor 488 (Santa Cruz Biotechnology, sc-365704 AF488, 1:50 for immunofluorescence); rabbit polyclonal antiendoA3 (Sigma Life Sciences, HPA039381, 1:1,000 for Western blotting); rabbit monoclonal anti-giantin (Institut Curie, recombinant proteins platform, A-R-R#05, 1:50 for immunofluorescence); mouse monoclonal anti-dynamin (BD Biosciences, 610245, 1:4,000 for Western blotting); mouse monoclonal anti-clathrin heavy chain for Western blotting (BD Biosciences, 610500, 1:5,000); mouse monoclonal anti-α-tubulin (Sigma, T5168, 1:5,000 for Western blotting); rabbit polyclonal anti-β1adrenergic receptor (Abcam, ab3442, 1:1000 for immunofluorescence); rabbit polyclonal anti-Galectin-8 (Biorbyt, orb216142, 1:1,000 for Western blotting); rabbit monoclonal anti-ezrin (R&D Systems, MAB72391, 1:1,000 for Western blotting); mouse monoclonal anti-GST (Invitrogen, MA4-004, 1:1,000 for Western blotting); unconjugated secondary antibodies or conjugated to Alexa Fluor 488, 546 or 647 (Thermo Fisher Scientific); anti-mouse and anti-rabbit secondary antibodies conjugated to horseradish peroxidase (Sigma and Dako, respectively). ..
Flow Cytometry:Article Title: Endophilin-A3 and Galectin-8 control the clathrin-independent endocytosis of CD166
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology Animals and other organisms Human research participants Clinical data Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used The following antibodies were purchased from the indicated suppliers: mouse monoclonal anti-CD166 clone 3A6 (Bio-Rad, MCA1926, 1:200 for immunofluorescence and flow cytometry on human cell lines); goat polyclonal anti-CD166 (R&D Systems, AF1172, 1:20 for immunofluorescence on mouse cell lines); mouse monoclonal anti-CD166 clone B-6 (Santa Cruz Biotechnology, sc-74558, 1:500 for Western blotting); mouse monoclonal anti-TfR (BD Biosciences, 555534, 1:1,000 for Western blotting); rabbit polyclonal anti-α-adaptin (Santa Cruz Biotechnology, sc-10761, 1:1,000 for Western blotting); rabbit monoclonal anti-EGFR (Cell Signaling Technology, 4267, 1:4,000 for Western blotting); rabbit monoclonal anti-endoA1 (Cell Signaling Technology, 3 nature research | reporting sum m ary O ctober 2018 65469, 1:100 for immunofluorescence and 1:1,000 for Western blotting); mouse monoclonal anti-endoA2 (Santa Cruz Biotechnology, sc-365704, 1:50 for immunofluorescence and 1:500 for Western blotting); mouse monoclonal anti-endoA2 coupled to Alexa Fluor 488 (Santa Cruz Biotechnology, sc-365704 AF488, 1:50 for immunofluorescence); rabbit polyclonal antiendoA3 (Sigma Life Sciences, HPA039381, 1:1,000 for Western blotting); rabbit monoclonal anti-giantin (Institut Curie, recombinant proteins platform, A-R-R#05, 1:50 for immunofluorescence); mouse monoclonal anti-dynamin (BD Biosciences, 610245, 1:4,000 for Western blotting); mouse monoclonal anti-clathrin heavy chain for Western blotting (BD Biosciences, 610500, 1:5,000); mouse monoclonal anti-α-tubulin (Sigma, T5168, 1:5,000 for Western blotting); rabbit polyclonal anti-β1adrenergic receptor (Abcam, ab3442, 1:1000 for immunofluorescence); rabbit polyclonal anti-Galectin-8 (Biorbyt, orb216142, 1:1,000 for Western blotting); rabbit monoclonal anti-ezrin (R&D Systems, MAB72391, 1:1,000 for Western blotting); mouse monoclonal anti-GST (Invitrogen, MA4-004, 1:1,000 for Western blotting); unconjugated secondary antibodies or conjugated to Alexa Fluor 488, 546 or 647 (Thermo Fisher Scientific); anti-mouse and anti-rabbit secondary antibodies conjugated to horseradish peroxidase (Sigma and Dako, respectively). ..
Article Title: Endophilin-A3 and Galectin-8 control the clathrin-independent endocytosis of CD166
Article Snippet: .. The following antibodies were purchased from the indicated suppliers: mouse monoclonal anti-CD166 clone 3A6 (Bio-Rad, MCA1926, 1:200 for immunofluorescence and flow cytometry on human cell lines); goat polyclonal anti-CD166 (R&D Systems, AF1172, 1:20 for immunofluorescence on mouse cell lines); mouse monoclonal anti-CD166 clone B-6 (Santa Cruz Biotechnology, sc-74558, 1:500 for Western blotting); mouse monoclonal anti-TfR (BD Biosciences, 555534, 1:1,000 for Western blotting); rabbit polyclonal anti-α-adaptin (Santa Cruz Biotechnology, sc-10761, 1:1000 for Western blotting); rabbit monoclonal anti-EGFR (Cell Signaling Technology, 4267, 1:4000 for Western blotting); rabbit monoclonal anti-endoA1 (Cell Signaling Technology, 65469, 1:100 for immunofluorescence and 1:1000 for Western blotting); mouse monoclonal anti-endoA2 (Santa Cruz Biotechnology, sc-365704, 1:50 for immunofluorescence and 1:500 for Western blotting); mouse monoclonal anti-endoA2 coupled to Alexa Fluor 488 (Santa Cruz Biotechnology, sc-365704 AF488, 1:50 for immunofluorescence); rabbit polyclonal anti-endoA3 (Sigma Life Sciences, HPA039381, 1:1,000 for Western blotting); rabbit monoclonal anti-giantin (Institut Curie, recombinant proteins platform, A-R-R#05, 1:50 for immunofluorescence); mouse monoclonal anti-dynamin (BD Biosciences, 610245, 1:4,000 for Western blotting); mouse monoclonal anti-clathrin heavy chain for Western blotting (BD Biosciences, 610500, 1:5000); mouse monoclonal anti-α-tubulin (Sigma, T5168, 1:5000 for Western blotting); rabbit polyclonal anti-β1-adrenergic receptor (Abcam, ab3442, 1:1000 for immunofluorescence); rabbit polyclonal anti-Galectin-8 (Biorbyt, orb216142, 1:1000 for Western blotting); rabbit monoclonal anti-ezrin (R&D Systems, MAB72391, 1:1000 for Western blotting); mouse monoclonal anti-GST (Invitrogen, MA4-004, 1:1000 for Western blotting); unconjugated secondary antibodies or conjugated to Alexa Fluor 488, 546, or 647 (Thermo Fisher Scientific); anti-mouse and anti-rabbit secondary antibodies conjugated to horseradish peroxidase (Sigma and Dako, respectively). ..
Magnetic Resonance Imaging:Article Title: Endophilin-A3 and Galectin-8 control the clathrin-independent endocytosis of CD166
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology Animals and other organisms Human research participants Clinical data Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used The following antibodies were purchased from the indicated suppliers: mouse monoclonal anti-CD166 clone 3A6 (Bio-Rad, MCA1926, 1:200 for immunofluorescence and flow cytometry on human cell lines); goat polyclonal anti-CD166 (R&D Systems, AF1172, 1:20 for immunofluorescence on mouse cell lines); mouse monoclonal anti-CD166 clone B-6 (Santa Cruz Biotechnology, sc-74558, 1:500 for Western blotting); mouse monoclonal anti-TfR (BD Biosciences, 555534, 1:1,000 for Western blotting); rabbit polyclonal anti-α-adaptin (Santa Cruz Biotechnology, sc-10761, 1:1,000 for Western blotting); rabbit monoclonal anti-EGFR (Cell Signaling Technology, 4267, 1:4,000 for Western blotting); rabbit monoclonal anti-endoA1 (Cell Signaling Technology, 3 nature research | reporting sum m ary O ctober 2018 65469, 1:100 for immunofluorescence and 1:1,000 for Western blotting); mouse monoclonal anti-endoA2 (Santa Cruz Biotechnology, sc-365704, 1:50 for immunofluorescence and 1:500 for Western blotting); mouse monoclonal anti-endoA2 coupled to Alexa Fluor 488 (Santa Cruz Biotechnology, sc-365704 AF488, 1:50 for immunofluorescence); rabbit polyclonal antiendoA3 (Sigma Life Sciences, HPA039381, 1:1,000 for Western blotting); rabbit monoclonal anti-giantin (Institut Curie, recombinant proteins platform, A-R-R#05, 1:50 for immunofluorescence); mouse monoclonal anti-dynamin (BD Biosciences, 610245, 1:4,000 for Western blotting); mouse monoclonal anti-clathrin heavy chain for Western blotting (BD Biosciences, 610500, 1:5,000); mouse monoclonal anti-α-tubulin (Sigma, T5168, 1:5,000 for Western blotting); rabbit polyclonal anti-β1adrenergic receptor (Abcam, ab3442, 1:1000 for immunofluorescence); rabbit polyclonal anti-Galectin-8 (Biorbyt, orb216142, 1:1,000 for Western blotting); rabbit monoclonal anti-ezrin (R&D Systems, MAB72391, 1:1,000 for Western blotting); mouse monoclonal anti-GST (Invitrogen, MA4-004, 1:1,000 for Western blotting); unconjugated secondary antibodies or conjugated to Alexa Fluor 488, 546 or 647 (Thermo Fisher Scientific); anti-mouse and anti-rabbit secondary antibodies conjugated to horseradish peroxidase (Sigma and Dako, respectively). ..
Immunofluorescence:Article Title: Endophilin-A3 and Galectin-8 control the clathrin-independent endocytosis of CD166
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology Animals and other organisms Human research participants Clinical data Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used The following antibodies were purchased from the indicated suppliers: mouse monoclonal anti-CD166 clone 3A6 (Bio-Rad, MCA1926, 1:200 for immunofluorescence and flow cytometry on human cell lines); goat polyclonal anti-CD166 (R&D Systems, AF1172, 1:20 for immunofluorescence on mouse cell lines); mouse monoclonal anti-CD166 clone B-6 (Santa Cruz Biotechnology, sc-74558, 1:500 for Western blotting); mouse monoclonal anti-TfR (BD Biosciences, 555534, 1:1,000 for Western blotting); rabbit polyclonal anti-α-adaptin (Santa Cruz Biotechnology, sc-10761, 1:1,000 for Western blotting); rabbit monoclonal anti-EGFR (Cell Signaling Technology, 4267, 1:4,000 for Western blotting); rabbit monoclonal anti-endoA1 (Cell Signaling Technology, 3 nature research | reporting sum m ary O ctober 2018 65469, 1:100 for immunofluorescence and 1:1,000 for Western blotting); mouse monoclonal anti-endoA2 (Santa Cruz Biotechnology, sc-365704, 1:50 for immunofluorescence and 1:500 for Western blotting); mouse monoclonal anti-endoA2 coupled to Alexa Fluor 488 (Santa Cruz Biotechnology, sc-365704 AF488, 1:50 for immunofluorescence); rabbit polyclonal antiendoA3 (Sigma Life Sciences, HPA039381, 1:1,000 for Western blotting); rabbit monoclonal anti-giantin (Institut Curie, recombinant proteins platform, A-R-R#05, 1:50 for immunofluorescence); mouse monoclonal anti-dynamin (BD Biosciences, 610245, 1:4,000 for Western blotting); mouse monoclonal anti-clathrin heavy chain for Western blotting (BD Biosciences, 610500, 1:5,000); mouse monoclonal anti-α-tubulin (Sigma, T5168, 1:5,000 for Western blotting); rabbit polyclonal anti-β1adrenergic receptor (Abcam, ab3442, 1:1000 for immunofluorescence); rabbit polyclonal anti-Galectin-8 (Biorbyt, orb216142, 1:1,000 for Western blotting); rabbit monoclonal anti-ezrin (R&D Systems, MAB72391, 1:1,000 for Western blotting); mouse monoclonal anti-GST (Invitrogen, MA4-004, 1:1,000 for Western blotting); unconjugated secondary antibodies or conjugated to Alexa Fluor 488, 546 or 647 (Thermo Fisher Scientific); anti-mouse and anti-rabbit secondary antibodies conjugated to horseradish peroxidase (Sigma and Dako, respectively). ..
Article Title: Endophilin-A3 and Galectin-8 control the clathrin-independent endocytosis of CD166
Article Snippet: .. The following antibodies were purchased from the indicated suppliers: mouse monoclonal anti-CD166 clone 3A6 (Bio-Rad, MCA1926, 1:200 for immunofluorescence and flow cytometry on human cell lines); goat polyclonal anti-CD166 (R&D Systems, AF1172, 1:20 for immunofluorescence on mouse cell lines); mouse monoclonal anti-CD166 clone B-6 (Santa Cruz Biotechnology, sc-74558, 1:500 for Western blotting); mouse monoclonal anti-TfR (BD Biosciences, 555534, 1:1,000 for Western blotting); rabbit polyclonal anti-α-adaptin (Santa Cruz Biotechnology, sc-10761, 1:1000 for Western blotting); rabbit monoclonal anti-EGFR (Cell Signaling Technology, 4267, 1:4000 for Western blotting); rabbit monoclonal anti-endoA1 (Cell Signaling Technology, 65469, 1:100 for immunofluorescence and 1:1000 for Western blotting); mouse monoclonal anti-endoA2 (Santa Cruz Biotechnology, sc-365704, 1:50 for immunofluorescence and 1:500 for Western blotting); mouse monoclonal anti-endoA2 coupled to Alexa Fluor 488 (Santa Cruz Biotechnology, sc-365704 AF488, 1:50 for immunofluorescence); rabbit polyclonal anti-endoA3 (Sigma Life Sciences, HPA039381, 1:1,000 for Western blotting); rabbit monoclonal anti-giantin (Institut Curie, recombinant proteins platform, A-R-R#05, 1:50 for immunofluorescence); mouse monoclonal anti-dynamin (BD Biosciences, 610245, 1:4,000 for Western blotting); mouse monoclonal anti-clathrin heavy chain for Western blotting (BD Biosciences, 610500, 1:5000); mouse monoclonal anti-α-tubulin (Sigma, T5168, 1:5000 for Western blotting); rabbit polyclonal anti-β1-adrenergic receptor (Abcam, ab3442, 1:1000 for immunofluorescence); rabbit polyclonal anti-Galectin-8 (Biorbyt, orb216142, 1:1000 for Western blotting); rabbit monoclonal anti-ezrin (R&D Systems, MAB72391, 1:1000 for Western blotting); mouse monoclonal anti-GST (Invitrogen, MA4-004, 1:1000 for Western blotting); unconjugated secondary antibodies or conjugated to Alexa Fluor 488, 546, or 647 (Thermo Fisher Scientific); anti-mouse and anti-rabbit secondary antibodies conjugated to horseradish peroxidase (Sigma and Dako, respectively). ..
Western Blot:Article Title: Endophilin-A3 and Galectin-8 control the clathrin-independent endocytosis of CD166
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology Animals and other organisms Human research participants Clinical data Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used The following antibodies were purchased from the indicated suppliers: mouse monoclonal anti-CD166 clone 3A6 (Bio-Rad, MCA1926, 1:200 for immunofluorescence and flow cytometry on human cell lines); goat polyclonal anti-CD166 (R&D Systems, AF1172, 1:20 for immunofluorescence on mouse cell lines); mouse monoclonal anti-CD166 clone B-6 (Santa Cruz Biotechnology, sc-74558, 1:500 for Western blotting); mouse monoclonal anti-TfR (BD Biosciences, 555534, 1:1,000 for Western blotting); rabbit polyclonal anti-α-adaptin (Santa Cruz Biotechnology, sc-10761, 1:1,000 for Western blotting); rabbit monoclonal anti-EGFR (Cell Signaling Technology, 4267, 1:4,000 for Western blotting); rabbit monoclonal anti-endoA1 (Cell Signaling Technology, 3 nature research | reporting sum m ary O ctober 2018 65469, 1:100 for immunofluorescence and 1:1,000 for Western blotting); mouse monoclonal anti-endoA2 (Santa Cruz Biotechnology, sc-365704, 1:50 for immunofluorescence and 1:500 for Western blotting); mouse monoclonal anti-endoA2 coupled to Alexa Fluor 488 (Santa Cruz Biotechnology, sc-365704 AF488, 1:50 for immunofluorescence); rabbit polyclonal antiendoA3 (Sigma Life Sciences, HPA039381, 1:1,000 for Western blotting); rabbit monoclonal anti-giantin (Institut Curie, recombinant proteins platform, A-R-R#05, 1:50 for immunofluorescence); mouse monoclonal anti-dynamin (BD Biosciences, 610245, 1:4,000 for Western blotting); mouse monoclonal anti-clathrin heavy chain for Western blotting (BD Biosciences, 610500, 1:5,000); mouse monoclonal anti-α-tubulin (Sigma, T5168, 1:5,000 for Western blotting); rabbit polyclonal anti-β1adrenergic receptor (Abcam, ab3442, 1:1000 for immunofluorescence); rabbit polyclonal anti-Galectin-8 (Biorbyt, orb216142, 1:1,000 for Western blotting); rabbit monoclonal anti-ezrin (R&D Systems, MAB72391, 1:1,000 for Western blotting); mouse monoclonal anti-GST (Invitrogen, MA4-004, 1:1,000 for Western blotting); unconjugated secondary antibodies or conjugated to Alexa Fluor 488, 546 or 647 (Thermo Fisher Scientific); anti-mouse and anti-rabbit secondary antibodies conjugated to horseradish peroxidase (Sigma and Dako, respectively). ..
Article Title: Endophilin-A3 and Galectin-8 control the clathrin-independent endocytosis of CD166
Article Snippet: .. The following antibodies were purchased from the indicated suppliers: mouse monoclonal anti-CD166 clone 3A6 (Bio-Rad, MCA1926, 1:200 for immunofluorescence and flow cytometry on human cell lines); goat polyclonal anti-CD166 (R&D Systems, AF1172, 1:20 for immunofluorescence on mouse cell lines); mouse monoclonal anti-CD166 clone B-6 (Santa Cruz Biotechnology, sc-74558, 1:500 for Western blotting); mouse monoclonal anti-TfR (BD Biosciences, 555534, 1:1,000 for Western blotting); rabbit polyclonal anti-α-adaptin (Santa Cruz Biotechnology, sc-10761, 1:1000 for Western blotting); rabbit monoclonal anti-EGFR (Cell Signaling Technology, 4267, 1:4000 for Western blotting); rabbit monoclonal anti-endoA1 (Cell Signaling Technology, 65469, 1:100 for immunofluorescence and 1:1000 for Western blotting); mouse monoclonal anti-endoA2 (Santa Cruz Biotechnology, sc-365704, 1:50 for immunofluorescence and 1:500 for Western blotting); mouse monoclonal anti-endoA2 coupled to Alexa Fluor 488 (Santa Cruz Biotechnology, sc-365704 AF488, 1:50 for immunofluorescence); rabbit polyclonal anti-endoA3 (Sigma Life Sciences, HPA039381, 1:1,000 for Western blotting); rabbit monoclonal anti-giantin (Institut Curie, recombinant proteins platform, A-R-R#05, 1:50 for immunofluorescence); mouse monoclonal anti-dynamin (BD Biosciences, 610245, 1:4,000 for Western blotting); mouse monoclonal anti-clathrin heavy chain for Western blotting (BD Biosciences, 610500, 1:5000); mouse monoclonal anti-α-tubulin (Sigma, T5168, 1:5000 for Western blotting); rabbit polyclonal anti-β1-adrenergic receptor (Abcam, ab3442, 1:1000 for immunofluorescence); rabbit polyclonal anti-Galectin-8 (Biorbyt, orb216142, 1:1000 for Western blotting); rabbit monoclonal anti-ezrin (R&D Systems, MAB72391, 1:1000 for Western blotting); mouse monoclonal anti-GST (Invitrogen, MA4-004, 1:1000 for Western blotting); unconjugated secondary antibodies or conjugated to Alexa Fluor 488, 546, or 647 (Thermo Fisher Scientific); anti-mouse and anti-rabbit secondary antibodies conjugated to horseradish peroxidase (Sigma and Dako, respectively). ..
Recombinant:Article Title: Endophilin-A3 and Galectin-8 control the clathrin-independent endocytosis of CD166
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology Animals and other organisms Human research participants Clinical data Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used The following antibodies were purchased from the indicated suppliers: mouse monoclonal anti-CD166 clone 3A6 (Bio-Rad, MCA1926, 1:200 for immunofluorescence and flow cytometry on human cell lines); goat polyclonal anti-CD166 (R&D Systems, AF1172, 1:20 for immunofluorescence on mouse cell lines); mouse monoclonal anti-CD166 clone B-6 (Santa Cruz Biotechnology, sc-74558, 1:500 for Western blotting); mouse monoclonal anti-TfR (BD Biosciences, 555534, 1:1,000 for Western blotting); rabbit polyclonal anti-α-adaptin (Santa Cruz Biotechnology, sc-10761, 1:1,000 for Western blotting); rabbit monoclonal anti-EGFR (Cell Signaling Technology, 4267, 1:4,000 for Western blotting); rabbit monoclonal anti-endoA1 (Cell Signaling Technology, 3 nature research | reporting sum m ary O ctober 2018 65469, 1:100 for immunofluorescence and 1:1,000 for Western blotting); mouse monoclonal anti-endoA2 (Santa Cruz Biotechnology, sc-365704, 1:50 for immunofluorescence and 1:500 for Western blotting); mouse monoclonal anti-endoA2 coupled to Alexa Fluor 488 (Santa Cruz Biotechnology, sc-365704 AF488, 1:50 for immunofluorescence); rabbit polyclonal antiendoA3 (Sigma Life Sciences, HPA039381, 1:1,000 for Western blotting); rabbit monoclonal anti-giantin (Institut Curie, recombinant proteins platform, A-R-R#05, 1:50 for immunofluorescence); mouse monoclonal anti-dynamin (BD Biosciences, 610245, 1:4,000 for Western blotting); mouse monoclonal anti-clathrin heavy chain for Western blotting (BD Biosciences, 610500, 1:5,000); mouse monoclonal anti-α-tubulin (Sigma, T5168, 1:5,000 for Western blotting); rabbit polyclonal anti-β1adrenergic receptor (Abcam, ab3442, 1:1000 for immunofluorescence); rabbit polyclonal anti-Galectin-8 (Biorbyt, orb216142, 1:1,000 for Western blotting); rabbit monoclonal anti-ezrin (R&D Systems, MAB72391, 1:1,000 for Western blotting); mouse monoclonal anti-GST (Invitrogen, MA4-004, 1:1,000 for Western blotting); unconjugated secondary antibodies or conjugated to Alexa Fluor 488, 546 or 647 (Thermo Fisher Scientific); anti-mouse and anti-rabbit secondary antibodies conjugated to horseradish peroxidase (Sigma and Dako, respectively). ..
Article Title: Endophilin-A3 and Galectin-8 control the clathrin-independent endocytosis of CD166
Article Snippet: .. The following antibodies were purchased from the indicated suppliers: mouse monoclonal anti-CD166 clone 3A6 (Bio-Rad, MCA1926, 1:200 for immunofluorescence and flow cytometry on human cell lines); goat polyclonal anti-CD166 (R&D Systems, AF1172, 1:20 for immunofluorescence on mouse cell lines); mouse monoclonal anti-CD166 clone B-6 (Santa Cruz Biotechnology, sc-74558, 1:500 for Western blotting); mouse monoclonal anti-TfR (BD Biosciences, 555534, 1:1,000 for Western blotting); rabbit polyclonal anti-α-adaptin (Santa Cruz Biotechnology, sc-10761, 1:1000 for Western blotting); rabbit monoclonal anti-EGFR (Cell Signaling Technology, 4267, 1:4000 for Western blotting); rabbit monoclonal anti-endoA1 (Cell Signaling Technology, 65469, 1:100 for immunofluorescence and 1:1000 for Western blotting); mouse monoclonal anti-endoA2 (Santa Cruz Biotechnology, sc-365704, 1:50 for immunofluorescence and 1:500 for Western blotting); mouse monoclonal anti-endoA2 coupled to Alexa Fluor 488 (Santa Cruz Biotechnology, sc-365704 AF488, 1:50 for immunofluorescence); rabbit polyclonal anti-endoA3 (Sigma Life Sciences, HPA039381, 1:1,000 for Western blotting); rabbit monoclonal anti-giantin (Institut Curie, recombinant proteins platform, A-R-R#05, 1:50 for immunofluorescence); mouse monoclonal anti-dynamin (BD Biosciences, 610245, 1:4,000 for Western blotting); mouse monoclonal anti-clathrin heavy chain for Western blotting (BD Biosciences, 610500, 1:5000); mouse monoclonal anti-α-tubulin (Sigma, T5168, 1:5000 for Western blotting); rabbit polyclonal anti-β1-adrenergic receptor (Abcam, ab3442, 1:1000 for immunofluorescence); rabbit polyclonal anti-Galectin-8 (Biorbyt, orb216142, 1:1000 for Western blotting); rabbit monoclonal anti-ezrin (R&D Systems, MAB72391, 1:1000 for Western blotting); mouse monoclonal anti-GST (Invitrogen, MA4-004, 1:1000 for Western blotting); unconjugated secondary antibodies or conjugated to Alexa Fluor 488, 546, or 647 (Thermo Fisher Scientific); anti-mouse and anti-rabbit secondary antibodies conjugated to horseradish peroxidase (Sigma and Dako, respectively). ..
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